For more information about making your library accessible to everyone, consult The Student Services Conference Room. It includes checklists for career services, distance learning, computer labs, recruitment and admissions, registration, housing and residential life, financial aid, libraries, tutoring and learning centers, and student organizations. The Student Services Conference Room also includes a searchable Knowledge Base of questions and answers, case studies, and promising practices.
HiI have many custom libraries or custom projects included in activity creator projects.Try to double click on .Activities.Design project and check if every dependency is properly included. For my custom project i added line here:
activity designer library vs activity library
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Each activity is required to have an Activity Monitoring, Evaluation, and Learning (MEL) Plan to support management of the activity and to inform USAID and the implementing partner about whether and how an activity is making progress toward stated results. This How-To Note provides an overview of what is typically included in an Activity MEL Plan and outlines practical steps for developing, reviewing, and maintaining one.
Every April, school librarians are encouraged to host activities and events to help their school and local community celebrate the vital role that strong school library programs play in transforming learning.
Celebrate Take Your Child to the Library Day with a special concert presented by Eric Ode. It's a library celebration filled with high-participation music, stories, and poems from a beloved author and award-wining songwriter. All children and their families are welcome to join the fun.
A Book Club of One's OwnSpecial Zoom Session with author Laurie Lico AlbaneseFebruary 23, 2023, 4:30-5:30 pmPlease join us in person or via Zoom to hear Laurie Lico Albanese chat about her books, creative process, publishing and other topics. There will be a viewing session at the library with refreshments and extra copies of Laurie's books available for check-out. If you can't make it to the library, but would like to watch the Zoom session, please register in advance. There is no cost for this event. Recommended for adults. Hope you are able to attend!
Our mission is to help make San Marcos a great place to live by providing our citizens with access to an outstanding collection of library materials; entertaining and educational programs for all ages; fast, free computer, Internet, and information services; and comfortable facilities in which to read, relax, and connect with others. We strive to provide truly outstanding customer service and to promote reading and learning as vital, enjoyable, lifelong activities.
The level of interactivity has a major impact on the quality of online learning courses and programs (Muirhead, 2001). Analyzing the types of interactions provided to the students can foster a deeper, more meaningful online learning experience.
Beanstack is the an online platform for reading and activity challenges. Create a family account to log reading and participate in various challenges. Mobile App: Google Play Apple App Store
As industry leaders in visioning, programming and planning for the library of the 21st century, we here at Margaret Sullivan Studio are often asked about the latest trends in library design. Certainly there are specifics, but ultimately, the trend is to design for positive experiences.
True flexibility in a library design results in a variety of spaces and places that foster activities, with select furnishings, technology and special equipment that enable a positive outcome. Toss in an appealing look and feel and you will have a 21st-century library that your community will love and call home.
Specifically, we have shifted from creating a program based on components (i.e., books, tables, chairs, computers, reader seats, lounge seats, etc.) to creating a library designed around spaces to foster activities and evoke positive feelings.
Incorporating placemaking principles, we have designed the 10 essential places in a public library to foster community. These places are scalable, and no matter the size, shape or location, your library can embody aspects of all of these components.
This is a place that is inviting, approachable on all sides, and full of information or people who know where to find it. The most invigorating welcome spaces are equipped with clear signage, retail-like displays and anything that promotes the activites and programs at the library.
Although we have designed and specified retail display furnishings, our clients have found that the most successful (and flexible) way to showcase new materials is to simply create attractive displays on tables. When located at the entry of the library, new books, fresh picks and current themes will just fly out of the library.
This is a flexible space that transforms based on new ideas that fill patrons with a sense of surprise and delight. Pop-ups can be characterized as any space, display or dedicated non-traditional furnishings that present library materials and programs in an unexpected way or allow librarians to experiment with non-traditonal services. Pop-ups can be anything from a bike cart to an outdoor reading garden to a dedicated room with rotating displays and programming.
These are outdoor spaces that invite relaxation, reflection, community activity and inspiration. Gardens can represent any outdoor programming space that allows the library building to mazimize its programming.
The Multnomah County library in Porland, Oregon has some very filthy tables and chairs as well as dirty keyboards for computer users. Sticky black dirt! Do the people who get jobs here think of anyone other than themselves. The library buildings are also dirty and stink. Remember to wash your hands when you are done.
Since then, another generation of high fidelity enzymes have been engineered using cell-based selection approaches. Yeast-based screening systems resulted in evolved Cas9 (evoCas9), which has four mutations in the REC3 domain. evoCas9 has less off-target activity than SpCas9-HF1 or eSpCas9(1.1). Phage-assisted continuous evolution (PACE) methods resulted in xCas9 3.7 which has 7 mutations found in the REC2, REC3, and PAM interacting domains and allows for expanded PAM recognition as well as increased specificity and lower off-target activity. By screening E. coli cells transformed with a pooled library of SpCas9 variants, researchers identified Sniper-Cas, which has less off-target activity than wild type Cas9 and is compatible with truncated gRNAs for increased specificity.
In the case of DNA libraries, the CRISPR library will be shipped at a concentration that is too low to be used in experiments. Thus, the first step in using your library is to amplify the library (panel C) to increase the total amount of DNA using the protocol specified by the depositing lab. When amplifying the library, it is important to maintain good representation of gRNAs so that the composition of your amplified library matches that of the original library. You'll use next-generation sequencing (NGS) to verify that this is the case. Learn more about library verification.
Once the library has been amplified/verified, the next step is to generate lentivirus containing the entire CRISPR library (panel D). Then, you will transduce cells with the lentiviral library (panel E). Remember - if you are using a 2-vector system, you will need to transduce cells that are already expressing Cas9. After applying your screening conditions, you will look for relevant genes (hits) using NGS technology. For more detail on using CRISPR for both positive and negative screens, see our pooled library guide.
Type VI CRISPR enzymes such as Cas13 recognize ssRNA rather than dsDNA. Many of these enzymes also have the ability to process crRNA precursors to mature crRNAs. Upon ssRNA recognition by the crRNA, the target RNA is degraded. In bacteria, Cas13 enzymes can also cleave RNAs non-specifically after the initial crRNA-guided cleavage. This promiscuous cleavage activity slows bacterial cell growth and may further protect bacteria from viral pathogens. Based on this feature, a Cas13a-based molecular detection platform, termed SHERLOCK, has been used to differentiate strains of Zika virus, genotype human DNA, and identify tumor mutations within cell-free genomic DNA. This non-specific cleavage does not occur in mammalian cells. Similar to Cas9 and Cas12 , Cas13 can be converted to an RNA-binding protein through mutation of its catalytic domain. Fusions to catalytically inactive Cas13 can be used to modulate RNA editing, for in vivo RNA imaging, or to control alternative splicing. Learn more about Cas13a or Cas13d.
To address these limitations, researchers have engineered SpCas9 enzymes with altered PAM specificities using a variety of approaches including phage-assisted evolution and directed mutagenesis. This resulted in the development of several SpCas9-derived variants with non-NGG PAM sequences. Another Cas9 alternative is xCas9, which targets a broad set of PAM sequences, such as NG, GAA, and GAT, while also displaying minimal off-target activity. SpCas9-NG, a variant that recognizes the NG PAM, has increased activity in vitro relative to other Cas9 endonucleases. Read more about Cas9 variants.
In order to limit off-target effects of CRISPR-Cas9, researchers use a class of small proteins called Acr (Anti-CRISPR) proteins to control CRISPR activity. Acr proteins are found in phages that have evolved ways to overcome the endogenous CRISPR systems used by various bacteria and archaea to protect against invading nucleic acids, such as phage genomes. Because of this diversity, Acr family members inhibit CRISPR by a variety of mechanisms. Some Acr proteins interfere with DNA binding at the PAM site whereas others interfere with the Cas9 HNH endonuclease domain. Furthermore, Acr proteins can be specific for a particular Cas9 species or can inhibit CRISPR enzymes across multiple bacterial species. For example, AcrIIA2 and AcrIIA4 isolated from Listeria monocytogenes can inhibit CRISPR activity of both LmoCas9 and SpCas9. 2ff7e9595c
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